Semaglutide reconstitution calculator.
Pre-filled with the 5 mg vial and 2 mL of bacteriostatic water the REGEN catalog reports for Semaglutide. Every field is editable.
The numbers for a 5 mg Semaglutide vial
Reconstituting a 5 mg vial with 2 mL of bacteriostatic water gives a concentration of 2.5 mg/mL. On a U-100 insulin syringe, where one unit is 0.01 mL, that puts 25 mcg in every unit drawn.
A 250 mcg dose at that concentration is 0.1 mL, which is 10 units on the syringe, and the vial holds about 20 of them.
Those are the values the calculator above opens with. The vial size and water volume are the amounts the REGEN catalog reports for Semaglutide; change any field and the arithmetic follows.
What the research reports for Semaglutide
Published protocols for Semaglutide report 0.25–2.4 mg, 1x weekly (titrated). That is what the literature describes, not a recommendation. The calculator starts at the low end of it. The Semaglutide reference page carries the evidence grade, the side-effect profile, and the primary sources behind that range.
Semaglutide reconstitution chart for a 5 mg vial
The same 5 mg of Semaglutide at four common water volumes. More water does not mean more compound, it spreads the same amount across a bigger volume, so the same dose is drawn to a higher number of units. That is usually the safer direction to be imprecise in: small errors on a longer draw matter proportionally less.
| Bacteriostatic water | Concentration | Per unit (U-100) | Units for 250 mcg |
|---|---|---|---|
| 1 mL | 5 mg/mL | 50 mcg | 5 units |
| 2 mL (catalog) | 2.5 mg/mL | 25 mcg | 10 units |
| 3 mL | 1.67 mg/mL | 16.7 mcg | 15 units |
| 5 mL | 1 mg/mL | 10 mcg | 25 units |
"Units" here means syringe graduations, not international units. They are unrelated measures that share a name, and conflating them is its own category of mistake. The reconstitution calculator explains the arithmetic behind these columns in full.
More on Semaglutide
The Semaglutide reference page covers how it works, what the research grade means, side effects, and pharmacokinetics, alongside the rest of the weight loss compounds. For a different compound, the generic reconstitution calculator takes any vial and dose.