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MOTS-c reconstitution calculator.

Pre-filled with the 10 mg vial and 2 mL of bacteriostatic water the REGEN catalog reports for MOTS-c. Every field is editable.

Syringe

The numbers for a 10 mg MOTS-c vial

Reconstituting a 10 mg vial with 2 mL of bacteriostatic water gives a concentration of 5 mg/mL. On a U-100 insulin syringe, where one unit is 0.01 mL, that puts 50 mcg in every unit drawn.

A 5000 mcg dose at that concentration is 1 mL, which is 100 units on the syringe, and the vial holds about 2 of them.

Those are the values the calculator above opens with. The vial size and water volume are the amounts the REGEN catalog reports for MOTS-c; change any field and the arithmetic follows.

What the research reports for MOTS-c

Published protocols for MOTS-c report 5–10 mg, 2-3x weekly. That is what the literature describes, not a recommendation. The calculator starts at the low end of it. The MOTS-c reference page carries the evidence grade, the side-effect profile, and the primary sources behind that range.

MOTS-c reconstitution chart for a 10 mg vial

The same 10 mg of MOTS-c at four common water volumes. More water does not mean more compound, it spreads the same amount across a bigger volume, so the same dose is drawn to a higher number of units. That is usually the safer direction to be imprecise in: small errors on a longer draw matter proportionally less.

Bacteriostatic waterConcentrationPer unit (U-100)Units for 5000 mcg
1 mL10 mg/mL100 mcg50 units
2 mL (catalog)5 mg/mL50 mcg100 units
3 mL3.33 mg/mL33.3 mcg150 units
5 mL2 mg/mL20 mcg250 units

"Units" here means syringe graduations, not international units. They are unrelated measures that share a name, and conflating them is its own category of mistake. The reconstitution calculator explains the arithmetic behind these columns in full.

More on MOTS-c

The MOTS-c reference page covers how it works, what the research grade means, side effects, and pharmacokinetics, alongside the rest of the longevity compounds. For a different compound, the generic reconstitution calculator takes any vial and dose.

Put the whole protocol
in one place.