Humanin reconstitution calculator.
Pre-filled with the 5 mg vial and 2 mL of bacteriostatic water the REGEN catalog reports for Humanin. Every field is editable.
The numbers for a 5 mg Humanin vial
Reconstituting a 5 mg vial with 2 mL of bacteriostatic water gives a concentration of 2.5 mg/mL. On a U-100 insulin syringe, where one unit is 0.01 mL, that puts 25 mcg in every unit drawn.
A 1000 mcg dose at that concentration is 0.4 mL, which is 40 units on the syringe, and the vial holds about 5 of them.
Those are the values the calculator above opens with. The vial size and water volume are the amounts the REGEN catalog reports for Humanin; change any field and the arithmetic follows.
What the research reports for Humanin
Published protocols for Humanin report 1–2 mg, 1x daily. That is what the literature describes, not a recommendation. The calculator starts at the low end of it. The Humanin reference page carries the evidence grade, the side-effect profile, and the primary sources behind that range.
Humanin reconstitution chart for a 5 mg vial
The same 5 mg of Humanin at four common water volumes. More water does not mean more compound, it spreads the same amount across a bigger volume, so the same dose is drawn to a higher number of units. That is usually the safer direction to be imprecise in: small errors on a longer draw matter proportionally less.
| Bacteriostatic water | Concentration | Per unit (U-100) | Units for 1000 mcg |
|---|---|---|---|
| 1 mL | 5 mg/mL | 50 mcg | 20 units |
| 2 mL (catalog) | 2.5 mg/mL | 25 mcg | 40 units |
| 3 mL | 1.67 mg/mL | 16.7 mcg | 60 units |
| 5 mL | 1 mg/mL | 10 mcg | 100 units |
"Units" here means syringe graduations, not international units. They are unrelated measures that share a name, and conflating them is its own category of mistake. The reconstitution calculator explains the arithmetic behind these columns in full.
More on Humanin
The Humanin reference page covers how it works, what the research grade means, side effects, and pharmacokinetics, alongside the rest of the longevity compounds. For a different compound, the generic reconstitution calculator takes any vial and dose.