NAD+ reconstitution calculator.
Pre-filled with the 500 mg vial and 5 mL of bacteriostatic water the REGEN catalog reports for NAD+. Every field is editable.
The numbers for a 500 mg NAD+ vial
Reconstituting a 500 mg vial with 5 mL of bacteriostatic water gives a concentration of 100 mg/mL. On a U-100 insulin syringe, where one unit is 0.01 mL, that puts 1000 mcg in every unit drawn.
A 50000 mcg dose at that concentration is 0.5 mL, which is 50 units on the syringe, and the vial holds about 10 of them.
Those are the values the calculator above opens with. The vial size and water volume are the amounts the REGEN catalog reports for NAD+; change any field and the arithmetic follows.
What the research reports for NAD+
Published protocols for NAD+ report 50–750 mg, route-dependent. That is what the literature describes, not a recommendation. The calculator starts at the low end of it. The NAD+ reference page carries the evidence grade, the side-effect profile, and the primary sources behind that range.
NAD+ reconstitution chart for a 500 mg vial
The same 500 mg of NAD+ at four common water volumes. More water does not mean more compound, it spreads the same amount across a bigger volume, so the same dose is drawn to a higher number of units. That is usually the safer direction to be imprecise in: small errors on a longer draw matter proportionally less.
| Bacteriostatic water | Concentration | Per unit (U-100) | Units for 50000 mcg |
|---|---|---|---|
| 1 mL | 500 mg/mL | 5000 mcg | 10 units |
| 2 mL | 250 mg/mL | 2500 mcg | 20 units |
| 3 mL | 166.67 mg/mL | 1666.7 mcg | 30 units |
| 5 mL (catalog) | 100 mg/mL | 1000 mcg | 50 units |
"Units" here means syringe graduations, not international units. They are unrelated measures that share a name, and conflating them is its own category of mistake. The reconstitution calculator explains the arithmetic behind these columns in full.
More on NAD+
The NAD+ reference page covers how it works, what the research grade means, side effects, and pharmacokinetics, alongside the rest of the recovery compounds. For a different compound, the generic reconstitution calculator takes any vial and dose.